ISO INTERNATIONAL STANDARD 18254-2 First edition 2018-12 Textiles Method for the detection and determination of alkylphenol ethoxylates (APEO) 一 Part 2: Method using NPLC Textiles-Methode de détection et dedetermination des alkylphenols éthoxylés (APEO) - Partie2:Méthode utilisant la CLPN Reference number IS0 18254-2:2018(E) ISO @ ISO 2018 IS018254-2:2018(E) COPYRIGHTPROTECTEDDOCUMENT IS02018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+4122749 0111 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2018 - All rights reserved IS0 18254-2:2018(E) Contents Page Foreword .iv Introduction ..V 1 Scope. ..1 2 Normative references ..1 3 Termsanddefinitions 4 Principle ..1 5 Reagents. ..1 6 Apparatus .2 7 Procedure .3 7.1 Standard preparation .3 7.2 Mobile phase for HPLC .3 7.2.1 Methanolwith0,1%formicacidand0,01%ammoniumformate 3 7.2.2 3 Acetonitrilewith0,1%formicacid 7.3 Sample preparation 3 7.4 Sample extraction. .3 7.5 Sample analysis 4 8 Calculationand calibration .4 8.1 Calculation of contributionpercentageof eachAPEO congenerin standard solution. .4 8.2 Calculationof concentrationofeachAPEO congenerin standardsolution 4 8.3 CalculationofconcentrationofeachAPEOcongenerinsample ..4 8.4 Calculationoftotal concentrationofallAPEOcongenerinsample .5 8.5 Calibration curve. .5 9 Test report ..5 Annex A (informative) Examples of chromatographic condition Annex B (informative) Examples of solidphaseextraction (SPE)procedurefor cleanup ..14 Annex C (informative) Results of the interlaboratory test .15 AnnexD (informative)Studyof the contributionpercentage (molefraction)of APEO congeners.16 Bibliography .18 IS0 2018 - All rights reserved ili